采用水热法制备Fe₃O₄纳米粒子，并用3-氨丙基三乙氧基硅烷（APTES）对其表面进行氨基化修饰。基于氧化石墨烯（GO）与氨基化Fe₃O₄的自组装体系，构建具有过氧化物模拟酶性能的磁性石墨烯复合纳米粒子（GO@Fe₃O₄），并对其理化性质进行表征，用于替代天然过氧化物酶快速检测样品中过氧化氢的含量。最优催化检测体系为：GO@Fe₃O₄固体粉末添加质量为0.005 g、反应温度为70℃、反应时间为50 min；在0.01~1.0 mmol/L过氧化氢浓度范围内，其浓度与检测体系吸光度呈现良好的线性关系，工作曲线为y=740.25x+0.079 8，相关系数R²=0.992 7，最低检出限为0.547 7 μmol/L，回收率为98.94%~105.74%。此外，所制备的磁性石墨烯复合纳米粒子模拟酶材料检测H₂O₂的体系具有较高的催化检测性能。

Fe₃O₄ nanoparticles are prepared by hydrothermal method,and their surfaces are modified by 3-aminopropyltriethyloxy silane (APTES) to form amino-coated Fe₃O₄ nanoparticles.Magnetic graphene composite nanoparticles (GO@Fe₃O₄) with peroxide-mimic enzyme properties are formed by graphene combined with amino-coated Fe₃O₄ through using self-assembly method,and their performance are characterized.GO@Fe₃O₄ are used to replace natural peroxidase to detect quickly the content of hydrogen peroxide in samples.The results show that the optimum detection system is as follow:the addition amount of GO@Fe₃O₄ solid powder is 0.005 g,the reaction temperature is set at 70℃ and the reaction time is 50 min.Standard working curve of H₂O₂ is a linear function of its concentration in the range of 0.01 to 1.0 mmol·L^-1,with a linearization equation of y=740.25x+0.079 8 and the correlation coefficient R² of 0.992 7.The lowest detection limit is 0.547 7 μmol·mL^-1 and the recovery rate is 98.94%-105.74%.In addition,the system for detecting H₂O₂ by magnetic graphene composite nanoparticles-mimic enzyme material has high catalytic detection performance.

龚德状(1995-),男,硕士研究生,研究方向为食品中有害物质检测分析,gongdezhuang1221@163.com